Which combination of restriction enzymes used for cloning a linear insert fragment into a digested plasmid vector has the least probability of generating self-ligated vectors following complete digestion and no further enzymatic treatment of the vector?

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Biology · Methods in Biology

Verified Solution4 OptionsUpdated: October 2026👁️1 View

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Insert digested with Hind III and Xho I; Vector digested with Hind III and Xho I

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Answer 1

Insert digested with Sma I; Vector digested with Sma I

Answer 2

Insert digested with Sma I and Hind III; Vector digested with Sma I

Answer 3

Insert digested with Hind III and Xho I; Vector digested with Hind III and Xho I

Answer 4

Insert digested with EcoR I; Vector digested with EcoR I

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