Biology · Microbiology
The nitrogen-fixing bacterium, Rhizobium leguminosarum, isolated from the root nodules of garden pea (Pisum sativum) is cultured in a petri plate containing appropriate nutrient agar medium. A bacterial colony was picked and inoculated into a liquid growth medium to scale up the culture for the production of biofertilizer. Which one of the following statements is correct?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1The rhizobial cells get transformed into bacteroids when grown in liquid media.
- 2The rhizobial cells cannot fix nitrogen when exposed to atmospheric air.✓
- 3The rhizobial cells when reinoculated into the rhizosphere of soybean plants will effectively nodulate its roots to fix atmospheric nitrogen.
- 4The liquid culture will be red/pink in color due to the accumulation of the pigment leghemoglobin.
Correct answer
The rhizobial cells cannot fix nitrogen when exposed to atmospheric air.
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