Biology · Biophysical Techniques
The Circular Dichroism (CD) spectra of a protein are recorded under two different environmental conditions. Under condition A, the spectrum exhibits two distinct negative minima at approximately 208 nm and 222 nm. Under condition B, these characteristic minima completely disappear, resulting in a single broad minimum around 200 nm. From the options given below, select the one that is the best interpretation of these spectra:
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1The difference between the spectra under conditions A and B is due to a lower protein concentration under condition B.
- 2The spectra represent the tertiary fold of the protein with condition A corresponding to a mixed α-helical + β-sheet fold and condition B corresponding to a largely β-sheet fold.
- 3The protein has an α-helical secondary structure under condition A that is converted to β-sheets under condition B.
- 4The protein has an α-helical secondary structure under condition A that is denatured under condition B.✓
Correct answer
The protein has an α-helical secondary structure under condition A that is denatured under condition B.
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