Biology · Biophysical Techniques
A cell lysate in 1% Triton X-100 was purified over an affinity column to isolate an active enzymatic complex. The purified complex was separated on a 10-50% continuous sucrose gradient. UV spectra of the fractions showed a strong absorbance peak at 260 nm that was significantly higher than the absorbance at 280 nm. Which of the following combinations of molecules would generate such spectra?
- 1Protein-Cholesterol
- 2Protein-Lipid
- 3Protein-RNA✓
- 4Protein-Protein
Correct answer
Protein-RNA
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