Biology · Life Processes
During the iodine test for starch in a leaf, the leaf is sequentially treated with boiling water, boiled in alcohol, and then dipped in iodine solution. What is the primary purpose of these biological steps and the resulting color change?
- 1Boiling breaks cell membranes, alcohol removes chlorophyll to decolorize the leaf, and iodine turns starch-containing regions blue-black.✓
- 2Boiling disinfects the leaf surface, alcohol permanently stains the leaf green, and iodine destroys accumulated starch.
- 3Boiling stiffens the cellular tissue, alcohol cleans oily residues, and iodine tests for the presence of proteins.
- 4Boiling softens the leaf structure, alcohol rapidly evaporates to dry it, and iodine enhances natural pigments.
Correct answer
Boiling breaks cell membranes, alcohol removes chlorophyll to decolorize the leaf, and iodine turns starch-containing regions blue-black.
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