ऑटोफैगी (autophagy) की प्रक्रिया के संबंध में निम्नलिखित कथनों पर विचार कीजिए: A. ऑटोफैगी तब होती है जब कोशिकाओं में एकत्रित प्रोटीन (aggregated proteins) होते हैं। B. ऑटोफैगोसोम किसी भी इंट्रासेल्यूलर ऑर्गेनेल के साथ गैर-विशिष्ट रूप से संलयित होते हैं। C. ऑटोफैगोसोम एक एकल-झिल्ली वाली संरचना है। D. ऑटोफैगोसोम लाइसोसोम के साथ संलयित होकर ऑटोफैगोलाइसोसोम बनाते हैं। उपरोक्त कथनों में से कौन सा संयोजन सही है?

Arrange the following events in the correct chronological order during receptor-mediated endocytosis of LDL and its subsequent intracellular fate: A. LDL binds to the LDL receptor in a clathrin-coated pit at the plasma membrane B. Dynamin oligomerises at the neck of the coated pit and hydrolyses GTP to pinch off the vesicle C. Clathrin triskelions are recruited via AP-2 adaptor binding to the NPXY internalisation signal of the LDL receptor D. Acidification of the early endosome (pH ~6) causes dissociation of LDL from its receptor E. LDL receptor is recycled to the plasma membrane via Rab11-positive recycling endosomes F. Cholesterol esters are hydrolysed by lysosomal acid lipase after late endosome–lysosome fusion

A researcher treats cultured mammalian cells with Brefeldin A (BFA) for 30 minutes and then labels newly synthesised proteins with 35S-methionine. She finds that: - Labelled proteins accumulate in the ER. - The cis-Golgi marker GM130 redistributes to the ER. - Lysosomal enzyme precursors (bearing mannose-6-phosphate) are secreted into the medium instead of reaching lysosomes. Which of the following mechanistic statements best explains all three observations?