Biology · Biotechnology
Which one of the following statements related to molecular cloning procedures is INCORRECT?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
Share:
- 1α-complementation/blue-white screening may produce blue coloured recombinant colonies (containing cloned fragments) in case of translational fusion with the β-galactosidase gene.
- 2To prevent self-ligation of a vector digested with Kpn I (GGTAC↓C), alkaline phosphatase enzyme is used to remove 3'-PO4 groups from the ends of fragments.✓
- 3A DNA fragment obtained as an Xho I fragment (C↓TCGAG) may be ligated at the Sal I site (G↓TCGAC) in a vector.
- 45' overhangs of restricted DNA fragments can be blunt-ended by Klenow polymerase but not by DNase I.
Correct answer
To prevent self-ligation of a vector digested with Kpn I (GGTAC↓C), alkaline phosphatase enzyme is used to remove 3'-PO4 groups from the ends of fragments.
Download Single Question PDF
Save this question and verified answer as a clean study sheet with live clickable link.
👥
Share with Friends & Study Groups
Challenge your peer aspirants on WhatsApp or Telegram to solve this question!
Online Test Series
Start Free Mock Test →Preparing for Biology?
Practice full-length mock tests with real exam timers, instant score analysis, and all-India ranking.
Chapter Practice
Practice Biotechnology MCQs →Want more practice questions on Biotechnology?
Solve complete topic-wise tests with detailed solutions on GkGet.
Related Practice Questions
Recommended practice questions in Biology · Biotechnology