Biology · Biotechniques
Which one of the following statements related to Agrobacterium-mediated genetic transformation of plants is correct?
- 1In a binary vector system of Agrobacterium tumefaciens, the oncogenes are deleted from the T-DNA, and vir genes are located on the helper plasmid.✓
- 2Agrobacterium-mediated transfer of T-DNA into a host plant does not require any host plant proteins.
- 3A transgenic plant containing two linked copies of the transgene in heterozygous cond ition would segregate in a3 : 1 ratio on self-pollination.
- 4Negative selection markers need to be expressed only under strong constitutive promoters for development of transgenic plants.
Correct answer
In a binary vector system of Agrobacterium tumefaciens, the oncogenes are deleted from the T-DNA, and vir genes are located on the helper plasmid.
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