Biology · Biotechnology
Which one of the following statements is CORRECT about Agrobacterium tumefaciens mediated plant transformation?
- 1In a binary vector system, the vir genes and two T-DNA border sequences (left and right) are present on the same transformation plasmid.
- 2Two T-DNA border sequences (left and right) are essential to design a binary vector system.✓
- 3The marker gene for the selection of bacterial transformants is located within the T-DNA segment of the transformation plasmid.
- 4The marker gene for the selection of plant transformants is located outside the T-DNA segment of the transformation plasmid.
Correct answer
Two T-DNA border sequences (left and right) are essential to design a binary vector system.
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