Biology · Immunological Techniques
Two different antibodies against the same protein were generated and named AB1 and AB2. AB1 was able to detect the target protein in Western blot, but not in ELISA. The opposite was true for AB2. Which one of the following is the most likely reason?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
Share:
- 1AB1 detects linear epitopes on unfolded protein, but AB2 detects conformational/discontinuous epitopes present on the folded protein.✓
- 2AB1 detects epitopes on the folded protein, whereas AB2 detects the unfolded epitopes.
- 3AB1 is unable to cross the cell membrane, whereas AB2 is membrane permeable.
- 4AB1 detects SDS-bound protein, whereas AB2 detects protein that is not bound to SDS.
Correct answer
AB1 detects linear epitopes on unfolded protein, but AB2 detects conformational/discontinuous epitopes present on the folded protein.
Download Single Question PDF
Save this question and verified answer as a clean study sheet with live clickable link.
👥
Share with Friends & Study Groups
Challenge your peer aspirants on WhatsApp or Telegram to solve this question!
Online Test Series
Start Free Mock Test →Preparing for Biology?
Practice full-length mock tests with real exam timers, instant score analysis, and all-India ranking.
Chapter Practice
Practice Immunological Techniques MCQs →Want more practice questions on Immunological Techniques?
Solve complete topic-wise tests with detailed solutions on GkGet.
Related Practice Questions
Recommended practice questions in Biology · Immunological Techniques