Biology · Cell Biology & Membrane Transport
To test if viral proteins are transported between Golgi stacks in vesicles or are released into the cytosol by one Golgi stack and taken up by another, viral protein transport was examined between Golgi stacks of infected and uninfected cells. Which one of the following experimental designs would best distinguish these two hypotheses?
- 1Test whether viral protein transport happens in the presence of antibodies depleting clathrin in acceptor Golgi stacks
- 2Test whether viral protein transport happens in the presence of antibodies depleting COPII in acceptor Golgi stacks
- 3Test whether viral protein transport happens in the presence of protease in the assay mix✓
- 4Test whether viral protein transport happens when either donor or acceptor Golgi stacks are treated with detergents sequentially
Correct answer
Test whether viral protein transport happens in the presence of protease in the assay mix
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