Biology · Methods in Biology

To investigate the relationship between microtubules and centrioles in fixed HeLa cells using an epifluorescence microscope, a researcher plans to conduct immunostaining using antibodies against tubulin and centrin (centriolar protein). Below is a list of secondary antibodies carrying various fluorophores (dyes) available to the researcher: A. Alexa 568 B. FITC C. Alexa 488 D. Alexa 647 Select the correct combination of dyes that the researcher would typically utilize to observe co-localization clearly without significant spectral overlap in a standard epifluorescence microscope.