Biology · Molecular Biology Techniques
To functionally characterize a newly identified gene suspected of encoding a K+ channel, a researcher would typically carry out which of the following steps? A. In vitro transcription of the cloned cDNA to produce the corresponding mRNA. B. Injecting the synthesized mRNA into Xenopus oocytes. C. Measuring channel-protein activity using the patch-clamp technique. D. Knocking out the homologous gene in oocytes and measuring activity via patch-clamp. Choose the correct combination of experiments that directly characterises the channel function from the cloned gene.
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1D, A and B
- 2C, D and A
- 3B, C and D
- 4A, B and C✓
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A, B and C
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