Biology · Cell Biology
To delineate the steps in endoplasmic reticulum (ER) transport, a student homogenized pancreatic acinar cells to isolate microsomes, which retain most of the biochemical properties of the ER. To verify that a protein is fully translocated into the lumen of the ER, the student planned several control experiments using combinations of protease and detergent treatments. Consider the following setups: A. Treat one set of microsomes first with detergent and then with protease. B. Treat one set of microsomes with protease only. C. Treat one set of microsomes with micrococcal nuclease. D. Treat one set of microsomes with detergent only. Select the option that represents the best combination of controls to confirm lumenal localization.
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1B and D only
- 2A and C only
- 3B, C and D only
- 4A, B and D only✓
Correct answer
A, B and D only
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