Biology · Biophysics
The theoretical resolution limit of the fluorescence microscope is about 200 nm. Super-resolution microscopy has been developed to address this limitation. Match the super-resolution microscopy methods in Column X with their principles in Column Y: Super-resolution microscopy (Column X) Principle (Column Y) A. Structured illumination microscopy (SIM) (I) Focused excitation laser point is surrounded by a donut-shaped depletion beam B. Stimulated emission depletion (STED) microscopy (II) The specimen is illuminated with a pattern of light and dark stripes to generate Moiré fringes C. Photoactivated localization microscopy (PALM) (III) Utilizes a variant of GFP that is activated by a wavelength different from its excitation wavelength Choose the correct match:
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1A - (II), B - (III), C - (I)
- 2A - (III), B - (II), C - (I)
- 3A - (II), B - (I), C - (III)✓
- 4A - (I), B - (II), C - (III)
Correct answer
A - (II), B - (I), C - (III)
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