Biology · Developmental Biology
The specification of sea urchin micromeres involves the activation of a repressor protein Pmar1, which represses the expression of hesC (which also encodes a repressor protein). One of the genes repressed by HesC is delta, whose expression is used as a marker for micromere lineage. In a normal embryo, whole mount in situ hybridization (WMISH) with a delta probe shows signal localized only to the micromeres (Area A), while the rest of the embryo (Area B) shows no signal. Consider the following experimental perturbations: A. WMISH with hesC probe in a normal embryo. B. WMISH with delta probe following microinjection of pmar1 mRNA into the fertilized egg. C. WMISH with delta probe following microinjection of antisense RNA to hesC into the fertilized egg. Match each experiment with the correct predicted spatial observation (i: Area A only; ii: Area B only; iii: Both Area A and B; iv: Neither Area A nor B).
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1(A - i), (B - ii), (C - iii)
- 2(A - i), (B - ii), (C - iv)
- 3(A - ii), (B - iii), (C - iv)
- 4(A - ii), (B - iii), (C - iii)✓
Correct answer
(A - ii), (B - iii), (C - iii)
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