Biology · Biochemical Techniques
The isoelectric points (pI) of four proteins (A, B, C, D) are 4.0, 5.5, 7.0, and 9.0 respectively. To purify protein 'D' from a mixture of these four proteins in a single step using ion-exchange chromatography, which combination of buffer pH and ion-exchange resin should be selected?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1pH 8, cation exchanger resin✓
- 2pH 6, anion exchanger resin
- 3pH 2, anion exchanger resin
- 4pH 11, cation exchanger resin
Correct answer
pH 8, cation exchanger resin
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