Biology · Biophysical Methods
The following statements were made about Laser Scanning Confocal Microscopy (LSCM): A. LSCM is a wide-field technique with a Kohler illumination system. B. Spatial resolution higher than that achieved in wide-field imaging could be obtained if only the central portion of an Airy Disk is used to form an image. C. Scanning mirrors sweep the excitation beam over the sample point-by-point to build the image. D. An altered pinhole size does not make any impact on the resolution of the image. E. A photomultiplier tube (PMT) in LSCM helps in generating real color of fluorophores. Which one of the following options represents the combination of all correct statements?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1B and E only
- 2B and C only✓
- 3C, D and E
- 4A, B and D
Correct answer
B and C only
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