Biology · Applied Biology

The following statements are made about transgenic mice technologies: A. Catalytically inactive Cas9 fused to a transcription activator can be used to activate specific gene expression. B. Using CRISPR/Cas9, a precise gene replacement in a target locus can be accomplished by non-homologous end joining repair. C. Using CRISPR/Cas9, many genes can be targeted at once by expressing many guide RNAs in the same cell. D. All chimeric mice derived by injecting engineered mouse embryonic stem cells into blastocysts will carry the transgene in the germ line. E. In the Cre-lox conditional system, the Cre recombinase is inserted into the gene to be knocked out. Which one of the following options represents the combination of all correct statements?