Biology · Enzyme Kinetics
The enzyme alkaline phosphatase was tested for its catalytic activity using the substrate para-nitrophenylphosphate. The KM obtained was 10 mM and Vmax was 100 μmol/min. Which one of the following options represents the initial velocity of the reaction at a substrate concentration of 10 mM?
- 120 μmol/min
- 2500 μmol/min
- 3100 μmol/min
- 450 μmol/min✓
Correct answer
50 μmol/min
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