Biology · Molecular Biology
The ColE1 plasmid has a low to medium copy number. However, pUC18, which is also a ColE1-based plasmid, has a high copy number because:
- 1It has a mutation in RNAII and the rop gene is overexpressed.
- 2It has a mutation in RNAI and the rop gene is overexpressed.
- 3It has a mutation in RNAII (primer for replication initiation) and does not carry the rop gene.
- 4It has a mutation in RNAI (antisense RNA) and does not carry the rop gene.✓
Correct answer
It has a mutation in RNAI (antisense RNA) and does not carry the rop gene.
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