Mathematics · Spectrophotometry Calculations
The absorbance of a protein solution at 280 nm was found to be 0.2 when measured in a 1 cm cuvette. What is the molar concentration of the protein if its molar extinction coefficient (ε280) is 10,000 M-1 cm-1?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 120 mM
- 220 μM✓
- 320 g L-1
- 420 mg/ml
Correct answer
20 μM
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