Biology · Microbiology
Tetracycline is commonly used as a selective agent in bacterial culture media during gene cloning experiments. What is its mechanism of action?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1It binds specifically to the 50S ribosomal subunit and prevents peptide bond formation.
- 2It blocks the protein initiation complex and causes misreading of the genetic code during translation.
- 3It binds to the 30S ribosomal subunit and prevents the binding of aminoacyl-tRNA to the A-site.✓
- 4It inhibits bacterial cell wall biosynthesis and is inactivated by β-lactamase.
Correct answer
It binds to the 30S ribosomal subunit and prevents the binding of aminoacyl-tRNA to the A-site.
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