Biology · Biotechnology Principles and Processes
Plasmid pBR322 has a Pst I restriction enzyme site within the ampR gene that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactosidase production and the recombinant plasmid is inserted into an E. coli strain, which of the following statements will be true?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1It will be able to produce a novel protein with dual ability.
- 2It will not be able to confer ampicillin resistance to the host cell.✓
- 3The transformed cells will have the ability to resist ampicillin as well as pro duce β-galactosidase.
- 4It will lead to the lysis of the host cell.
Correct answer
It will not be able to confer ampicillin resistance to the host cell.
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