Biology · Biotechnology

Inverse PCR is performed for site-directed mutagenesis with complementary primers (having the desired mutation) using a plasmid having the cloned gene as a template. The following statements were made regarding t his experiment: A. PCR is followed by transformation of bacterial cells directly with the reaction mixture. A large number of the transformants will have the wild type gene. B. The PCR mixture is treated with Dpn I and then used to transform bacterial cells. Most of the transformants will have the mutant gene. C. PCR is followed by transformation of bacterial cells directly with the reaction mixture. None of the transformants will have the mutant gene. D. The PCR mixture is treated with Dpn I and then used to transform bacterial cells. Half of the transformants will have the mutant gene. Which one of the following options represents a combination of all correct statements?