Biology · Biotechnology: Principles and Processes
In recombinant DNA technology, several sequential steps are involved. Arrange the following in the correct chronological order: 1. Isolation of the desired DNA fragment/gene2. Insertion of the gene into a suitable plasmid vector3. Introduction of recombinant DNA into the host cell (transformation)4. Selection of transformed host cells and identification of the clone containing the desired gene5. Expression/multiplication of the introduced gene in the host
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- 12 → 1 → 4 → 3 → 5
- 21 → 2 → 3 → 4 → 5✓
- 33 → 2 → 1 → 5 → 4
- 41 → 3 → 2 → 4 → 5
Correct answer
1 → 2 → 3 → 4 → 5
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