Biology · Immunology
In flow cytometry analysis of peripheral white blood cells, if R1 in the forward scatter (FSC) vs. side scatter (SSC) plot represents the selection window for lymphocytes, which of the following options correctly identifies the cell population in R1 and its segregation into quadrants based on CD4 and CD8 immunostaining?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1Plot 1 → lymphocytes; Plot 2 → (A) CD4 - CD8 - , (B) CD4 - CD8 + , (C) CD4 + CD8 - , (D) CD4 + CD8 +✓
- 2Plot 1 → neutrophils; Plot 2 → (A) CD4 + CD8 + , (B) CD4 - CD8 + , (C) CD4 + CD8 - , (D) CD4 + CD8 +
- 3Plot 1 → lymphocytes; Plot 2 → (A) CD8 + CD4 - , (B) CD4 + CD8 - , (C) CD4 - CD8 - , (D) CD8 + CD4 -
- 4Plot 1 → lymphocytes; Plot 2 → (A) CD4 - CD8 + , (B) CD4 - CD8 + , (C) CD4 + CD8 - , (D) CD8 + CD4 -
Correct answer
Plot 1 → lymphocytes; Plot 2 → (A) CD4 - CD8 - , (B) CD4 - CD8 + , (C) CD4 + CD8 - , (D) CD4 + CD8 +
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