Biology · Molecular Basis of Inheritance
In an experiment, E. coli cells grown in a medium containing 14NH4Cl (14N is the light isotope of nitrogen) are transferred and grown for six generations in a medium containing the heavy isotope of nitrogen (15N). After six generations, their DNA was extracted and subjected to CsCl density gradient centrifugation. Identify the types of DNA bands obtained and their corresponding ratio.
- 1Light : Heavy, 1 : 15
- 2Hybrid : Heavy, 1 : 31✓
- 3Light : Heavy, 1 : 31
- 4Hybrid : Heavy, 1 : 16
Correct answer
Hybrid : Heavy, 1 : 31
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