Biology · Biotechnology
In Agrobacterium-mediated transformation, which one of the following approaches is more likely to generate transgenic plants with INCOMPLETE transfer of the passenger gene?
- 1Expression of both selection marker gene and passenger gene under constitutive promoters
- 2Placement of passenger gene towards left border and marker gene towards right border of T-DNA✓
- 3Expression of selection marker gene under constitutive promoter and passenger gene under tissue-specific promoter
- 4Placement of selection marker gene towards left border and passenger gene towards right border of T-DNA
Correct answer
Placement of passenger gene towards left border and marker gene towards right border of T-DNA
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