In Agrobacterium-mediated genetic transformation designed to silence an endogenous constitutively expressed plant gene 'XYFP', expression cassettes within the T-DNA region can be engineered. To effectively achieve post-transcriptional gene silencing (PTGS) via RNA interference (RNAi), which of the following structural configurations within the construct must be utilized?

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Biology · Plant Biotechnology

Verified Solution4 OptionsUpdated: October 2026👁️1 View

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A construct containing inverted repeats of the XYFP gene fragment separated by a spacer/intron to form a hairpin RNA (hpRNA)

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Answer 1

Only a single sense strand expression cassette of XYFP

Answer 2

Only a single antisense strand expression cassette of XYFP

Answer 3

A construct that does not include a selectable marker gene cassette

Answer 4

A construct containing inverted repeats of the XYFP gene fragment separated by a spacer/intron to form a hairpin RNA (hpRNA)

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