Chemistry · Analytical Techniques
In affinity chromatography, retention of biomolecules in the column occurs mainly because of:
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1Partitioning between stationary and mobile phases based on polarity
- 2Variation in solubility of the solutes
- 3Reversible interactions with a specific ligand✓
- 4Differences in molecular size
Correct answer
Reversible interactions with a specific ligand
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