Biology · Recombinant DNA Technology
In a Southern blot analysis performed to identify T-DNA integration events among transgenic plants, a single band of hybridization indicates a single copy integration event. If the restriction enzyme used cuts only once within the T-DNA region, which of the following patterns on the autoradiogram would represent potential single copy integration events?
- 1Plants showing a single distinct band✓
- 2Plants showing two distinct bands
- 3Plants showing three distinct bands
- 4Plants showing no bands at all
Correct answer
Plants showing a single distinct band
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