Biology · Biotechnology and Transgenics
In a Southern blot analysis of five independent transgenic lines (A to E) developed using a transgene construct digested with a restriction enzyme that cuts once on each side of the probed gene, a single hybridizing band indicates a single-copy insertion event. If lines A, B, C, and D show multiple bands, and line E shows exactly one band, which option represents the single insertion transgenic events?
- 1B and C only
- 2A, B and E only
- 3A and B only
- 4E only✓
Correct answer
E only
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