Biology · Biophysical Chemistry
In a protein stability study, three solutions, MolA (10 kDa) at 0.5 mM, MolB (20 kDa) at 0.5 mM, and MolC (20 kDa) at 1 mM, were subjected to denaturation by urea, SDS, and guanidinium hydrochloride (GnHCl), respectively. The fraction of unfolded protein (f u ) profiles show that f u is 0.2 for MolA at 0.2 M urea, 0.8 for MolB at 0.05% SDS, and 0.9 for MolC at 4.5 M GnHCl. Which one of the following corresponds to reaction conditions at which the absolute number of molecules of folded protein are equal, assuming the reaction volumes to be the same for all experiments?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 10.2 M urea; 0.05% SDS; 4.5 M GnHCl✓
- 22 M urea; 0.05% SDS; 1 M GnHCl
- 30.2 M urea; 0.25% SDS; 4.5 M GnHCl
- 42 M urea; 0.25% SDS; 1 M GnHCl
Correct answer
0.2 M urea; 0.05% SDS; 4.5 M GnHCl
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