Biology · Recombinant DNA Technology

In a molecular cloning experiment, a 1 kb DNA insert containing a single asymmetric BamHI restriction site (located 200 bp from the 5' end and 800 bp from the 3' end) is cloned into the unique blunt-end SmaI site of a 3 kb vector. To confirm successful insertion and establish the specific orientation where the 5' end of the insert points toward a vector reference marker 'P', clones are digested with BamHI. Which of the following restriction digestion fragment profiles uniquely confirms this correct orientation?