Biology · Recombinant DNA Technology
In a cloning experiment, a DNA molecule isolated as a Pst I fragment was inserted into the Pst I site of a cloning vector that is 3 kb in length. Digestion of the CONFIRMED clones with Pst I resulted in the appearance of a single band corresponding to 3 kb in an agarose gel. Based on this information, what is the probable size of the cloned fragment?
- 14.5 kb
- 23 kb✓
- 31.5 kb
- 46 kb
Correct answer
3 kb
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