Biology · Biochemistry
Heating of some nucleic acids shows a hyperchromic effect characterized by an increase in the absorbance at 260 nm (A260). The sharp transition midpoint is defined as the melting temperature (Tm). Which one of the following nucleic acid samples is NOT expected to generate such a typical cooperative melting profile upon heating?
- 1Single-stranded DNA having imperfect random secondary structures✓
- 2DNA:RNA hybrid
- 3Double-stranded RNA
- 4Double-stranded DNA
Correct answer
Single-stranded DNA having imperfect random secondary structures
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