Given below are the restriction profiles obtained upon digestion of a 4.8 kb plasmid with four different enzymes (E1, E2, E3, and E4): E1: 4.8 kb; E1 + E2 = 500 bp + 4.3 kb E2: 4.8 kb; E2 + E3 = 400 bp + 4.4 kb E3: 4.8 kb; E3 + E4 = 2 kb + 2.8 kb E4: 4.8 kb; E1 + E4 = 1.9 kb + 2.9 kb Based on the above information, which one of the following statements is correct?

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Biology · Biotechnology

Verified Solution4 OptionsUpdated: October 2026👁️1 View

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Correct answer

E2 recognition sequence is located between E1 and E3.

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Answer 1

The sequence of recognition sites after digestion of the plasmid with E2 is: E1 - E3 - E4.

Answer 2

The sequence of recognition sites after digestion of the plasmid with E3 is: E4 - E2 - E1.

Answer 3

E2 recognition sequence is located between E1 and E3.

Answer 4

E1 and E4 have two recognition sites each in the plasmid.

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