Biology · Recombinant DNA Technology
Given below are statements related to various molecular techniques: A. During molecular cloning of DNA fragments, a vector and insert molecule digested with two different enzymes can never be ligated with each other. B. Only 3' → 5' exonucleases and not 5' → 3' exonucleases can be used for digesting nucleic acids to generate blunt-ended fragments for cloning. C. In Sanger's dideoxy sequencing method, each reaction consists of a mixture of three dNTPs and one ddNTP. D. Self-ligation of a vector with compatible ends can be prevented by treatment with alkaline phosphatase. Which one of the following options represents a combination of correct statements?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1A and B
- 2C and D
- 3D only✓
- 4B and C
Correct answer
D only
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