Biology · Biotechnology
Given below are some statements with blank spaces indicated by '_________'. A. A plasmid cloning vector digested with an enzyme (with a single restriction site in the plasmid) that generates 3' overhangs can be made blunt-ended using _________. B. DNA with a nucleotide composition of 30% A, 35% G, 20% C and 15% T is most likely _________. C. Production of only truncated molecules of transgene-derived mRNA in transgenic plants generated using a transgene from a prokaryotic source is most likely due to . D. _________ is a method for identifying the positions where individual DNA-binding proteins attach to a genome. Which one of the following options has the correct sequence of terms that can be used to complete the above statements (from A to D) such that all statements become true?
- 1A - Reverse transcriptase; B - single-stranded; C - absence of polyA signal; D - PFGE
- 2A - Mung bean nuclease; B - single-stranded; C - presence of potential poly-adenylation signals in the transgene sequence; D - Ch IP-seq✓
- 3A - Pfu polymerase; B - double-stranded; C - codon usage variations; D - ChIP-seq
- 4A - Taq polymerase; B - single-stranded; C - presence of mRNA instability sequences; D - FISH
Correct answer
A - Mung bean nuclease; B - single-stranded; C - presence of potential poly-adenylation signals in the transgene sequence; D - Ch IP-seq
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