Biology · Polymerase Chain Reaction (PCR)

Given below are four DNA sequences and a set of forward and reverse primers for PCR amplification: A. Sequence: 5'-ACAATCGT.....ACTAGTAC-3' Primers: FP: 5'-TGTTAG-3', RP: 5'-TAGTAC-3' B. Sequence: 5'-AGTCTTAG.....ATGCCAGT-3' Primers: FP: 5'-AAGACT-3', RP: 5'-ACTGGC-3' C. Sequence: 5'-CTTGACTA.....GTACAGTCA-3' Primers: FP: 5'-CTTGAC-3', RP: 5'-TGACTG-3' D. Sequence: 5'-GATCTAGC.....TCAAGCAGAC-3' Primers: FP: 5'-GATCTA-3', RP: 5'-CAGACG-3' In the absence of any other factors such as T m , length, percent GC, etc., which one of the above template-primer combinations would produce an amplified fragment?