Biology · Biotechnology

Given below are a few statements on transgenic plants: A. Transgenic plants generated using a transformation vector with the CaMV35S promoter-GUS-35SpA cassette can show variations in expression levels of GUS protein in independent transgenic events due to differences in strength of promoter used to express the GUS gene. B. A transgenic plant containing two insertions of the transgene cassette as inverted repeats in tandem would segregate in a 3 : 1 ratio for the transgenic phenotype on backcrossing the transgenic plant with the untransformed parent. C. A transgene containing a potential polyadenylation signal in its coding sequence would generate full-length transgene mRNA but a truncated transgenic protein. D. A gene-pyramiding experiment to bring together two transgenic traits by crossing independent homozygous single-copy transgenic lines for each trait would produce a plant homozygous for both the transgenes in the F2 generation. Which one of the following options represents a combination of only correct statements?