Biology · Methods in Biology
For the template sequence given below, which one of the following combinations of primers can hypothetically be used to amplify the target region? 5'- A T C G A C T A G NNNNNNNNNNNNNNNNN C C T A A T G C A G - 3'
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1Primer 1: 5'- TAGCTG - 3'; Primer 2: 5'- CTGCAT - 3'
- 2Primer 1: 5'- ATCGAC - 3'; Primer 2: 5'- GACGTA - 3'
- 3Primer 1: 5'- ATCGAC - 3'; Primer 2: 5'- CTGCAT - 3'✓
- 4Primer 1: 5'- TAGCTG - 3'; Primer 2: 5'- GACGTA - 3'
Correct answer
Primer 1: 5'- ATCGAC - 3'; Primer 2: 5'- CTGCAT - 3'
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