Biology · Genetics and Gene Mapping
Escherichia coli cells were simultaneously infected with two strains of phage λ. One strain of λ had a mutant host range, is temperature sensitive, and is known to produce clear plaques (genotype h st c); another strain of λ carried the wild-type alleles (genotype h + st + c + ). Progeny phages were collected from the lysed cells and plated on bacteria. The following numbers of different progeny plaques were obtained: h + c + st + : 350 h + c st : 86 h + c + st : 4 h c st : 300 h + c st + : 90 h c st + : 6 h c + st + : 114 h c + st : 50 What is the correct order of the three genes and the map distance between them?
- 1h - 36 cM - c - ∞ cM - st
- 2h - 21 cM - st - 15 cM - c✓
- 3c - 21 cM - h - 15 cM - st
- 4h - 36 cM - c - 15 cM - st
Correct answer
h - 21 cM - st - 15 cM - c
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