During the separation of proteins using discontinuous SDS-polyacrylamide gel electrophoresis (SDS-PAGE), what is the correct relative migration speed order of the major ionic species in the stacking gel that enables isotachophoresis?

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Biology · Biochemical Techniques

Verified Solution4 OptionsUpdated: October 2026👁️1 View

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Chloride ion moves faster than glycine, and glycine moves faster than the protein-SDS complex

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Answer 1

Chloride ion moves faster than glycine, and glycine moves faster than the protein-SDS complex

Answer 2

The protein-SDS complex moves faster than both chloride ion and glycine

Answer 3

Glycine moves faster than chloride ion and the protein-SDS complex

Answer 4

Chloride ion moves faster than the protein-SDS complex, and the complex moves faster than glycine

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