Biology · Molecular Biology
DNA polymerase PolA has high fidelity but low processivity, and DNA polymerase PolB has low fidelity and high processivity. In vitro reactions for DNA synthesis using limiting amounts of PolA or PolB were set up to further characterize the enzymes according to the following scheme: Tube 1: PolA + T7 DNA (20 min) + T3 DNA (40 min) Tube 2: PolB + T7 DNA (20 min) + T3 DNA (40 min) Which of the following outcomes do you expect?
- 1Both tubes will have more T7 DNA than T3 DNA.
- 2Both tubes will have more T3 DNA than T7 DNA.
- 3Tube 1 will have more T7 DNA and tube 2 will have more T3 DNA.
- 4Tube 1 will have more T3 DNA and tube 2 will have more T7 DNA.✓
Correct answer
Tube 1 will have more T3 DNA and tube 2 will have more T7 DNA.
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