Biology · Immunology
Different experimental approaches were used to quantify serum levels of IL-17 in human patient samples. Which one of the following approaches provides the most accurate quantification in a standard clinical s etting?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1Sandwich ELISA with monoclonal capture and detection antibodies against different epitopes of human IL-17✓
- 2Direct ELISA by coating the plate with patient serum and detection with polyclona l anti-human IL-17 antibody
- 3Fractionation of the serum sample on SDS-PAGE followed by Western blotting with polyclonal anti-human I L-17 antibody
- 4Sandwich ELISA, with monoclonal capture and detection antibodies against the same epitope of human IL-17
Correct answer
Sandwich ELISA with monoclonal capture and detection antibodies against different epitopes of human IL-17
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