Biotechnology · Recombination Systems
Consider two genetic constructs A and B introduced into eukaryotic cells. Construct A contains homotypic loxP sites flanking a fluorophore cassette, whereas construct B contains heterotypic loxP and loxN sites. Cre recombinase acts upon loxP-loxP (causing excision or inversion depending on orientation) and loxN-loxN sites. Which statement correctly describes the outcome upon Cre induction?
Verified Solution4 OptionsUpdated: October 2026👁️1 View
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- 1The cells transfected with construct B will yield two types of cells: each type expressing either red or yellow fluorescence.
- 2The cells transfected with construct A will yield two types of cells: each type expressing either red or green fluorescence.
- 3The cells transfected with construct B will yield three types of cells: each type expressing either red or green or yellow fluorescence.
- 4The cells transfected with construct A will yield three types of cells: each type expressing either red or green or yellow fluorescence.✓
Correct answer
The cells transfected with construct A will yield three types of cells: each type expressing either red or green or yellow fluorescence.
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