Biology · Transgenic Technology

Consider the following steps involved in developing a transgenic mouse via microinjection: C. The desired gene is preferably microinjected into the male pronucleus after sperm entry into the oocyte. E. Blastocyst stage embryos are transferred to the uterus of a hormonally prepared pseudopregnant mother. G. The female mouse of a specific strain is superovulated, oocytes are collected and allowed to fertilize in vitro. Which of the following combinations represents a valid chronological sequence of these critical stages in the development of a transgenic mouse?