Biology · Molecular Biology Techniques
Consider the following statements regarding Polymerase Chain Reaction (PCR): 1. PCR requires a thermostable DNA polymerase enzyme. 2. Synthetic oligonucleotide primers determine the specificity of the amplification. 3. PCR can be used directly for high-throughput automated Sanger sequencing without isolation. 4. Real-time PCR (qPCR) allows for the quantitative evaluation of target DNA. Which of the statements given above are correct?
- 11, 2, 3, and 4
- 22 and 4 only
- 31 and 3 only
- 41, 2, and 4 only✓
Correct answer
1, 2, and 4 only
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